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Part:BBa_K762500:Design

Designed by: Menuka Samaranayake   Group: iGEM12_Wisconsin-Madison   (2012-07-31)


Beta-glucosidase from Streptomyces coelicolor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1120
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 536
    Illegal AgeI site found at 151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 793


Design Notes

There is an internal Not1 site so the prefix and suffix are RFC 54 compliant.


Source

Amplified off of genomic DNA from Weibel Lab at UW Madison

References